Software error:
Can't call method "errstr" on an undefined value at /websites/parts.igem.org/cgi/lib/UNGDB.pm line 33.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Status: 500 Content-type: text/html
Software error:
Can't call method "errstr" on an undefined value at /websites/parts.igem.org/cgi/lib/BBDB.pm line 35. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Plate.pm line 13. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Plate.pm line 13. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Well.pm line 11. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Well.pm line 11. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Blast.pm line 15. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Blast.pm line 15. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Sample.pm line 24. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Sample.pm line 24. Compilation failed in require at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/WrapPart.pm line 10. Compilation failed in require at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 12. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/BBWeb.pm line 12. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Change.pm line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Change.pm line 8. Compilation failed in require at /websites/parts.igem.org/cgi/lib/Part.pm line 12. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/lib/Part.pm line 12. Compilation failed in require at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8. BEGIN failed--compilation aborted at /websites/parts.igem.org/cgi/partsdb/putout.cgi line 8.
For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.
Prlux-rbs-rfp-Ter.
It produces rfp when there is enough luxR and AHL.
From left to right, Marker(DL10000), Prlux, Prlux-rbs-rfp, Prlux-rbs-rfp-Ter, Prlux-rbs-rfp-rbs-luxI, Prlux-rbs-rfp-rbs-luxI-Ter, Prlux-rbs-rfp-rbs-luxR-Ter, Prlux-rbs-rfp-luxR, Prlux-rbs-rfp-luxI-rbs-luR, Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter.
The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//direction/forward
//function/cellsignalling/LuxR
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//function/cellsignalling/LuxR
//promoter
//regulation/positive
//rnap/prokaryote/ecoli/sigma70
| control | luxR, HSL |
| direction | Forward |
| negative_regulators | |
| o_h | |
| o_l | |
| positive_regulators | 1 |